کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8500256 1553730 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid detection of Trichinella spiralis larvae in muscles by loop-mediated isothermal amplification
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Rapid detection of Trichinella spiralis larvae in muscles by loop-mediated isothermal amplification
چکیده انگلیسی
► Sixteen sets of primers were designed to recognise a 1.6 kb repetitive element of the Trichinella spiralis genome. ► The sensitivity of LAMP with detection limits of 362 fg/μl is >10 times higher than that of PCR. ► The assay was able to detect T. spiralis in all mouse muscle samples infected with 10 T. spiralis larvae on day 20 p.i. ► We believe this is the first report on application of a LAMP assay for detection of T. spiralis larvae in muscle samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 42, Issues 13–14, December 2012, Pages 1119-1126
نویسندگان
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