Flow cytometry-assisted rapid isolation of recombinant Plasmodium berghei parasites exemplified by functional analysis of aquaglyceroporin

► Rapid, robust isolation of recombinant Plasmodium berghei lines by flow cytometry. ► Reduction by >80% of animal use for the generation of mutant parasite lines. ► Plasmodium berghei aquaglyceroporin is dispensable for all life cycle stages. ► Tagged aquaglyceroporin localizes to perinuclear structures inside the parasite.