کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8500322 | 1553734 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV](/preview/png/8500322.png)
چکیده انگلیسی
Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 106C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 38, Issues 8â9, July 2008, Pages 1051-1055
Journal: International Journal for Parasitology - Volume 38, Issues 8â9, July 2008, Pages 1051-1055
نویسندگان
M.C. Jenkins, J. Higgins, J.E. Abrahante, K.E. Kniel, C. O'Brien, J. Trout, C.A. Lancto, M.S. Abrahamsen, R. Fayer,