کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8500378 1553736 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loop 1 structure of the leishmanial ICAM-L molecule is crucial for parasite binding and infection of host macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Loop 1 structure of the leishmanial ICAM-L molecule is crucial for parasite binding and infection of host macrophages
چکیده انگلیسی
The binding of each intercellular adhesive molecule (ICAM) molecule fragment from Leishmania amazonensis (ICAM-L) to host macrophages was investigated using an indirect immunofluorescent sandwich technique, based on the observation that ICAM-L can block the uptake of L. amazonensis on the macrophage surface and all prepared ICAM-L fragments can react with rabbit anti-ICAM-L antiserum. The ICAM-L fragments lacking the loop 1 (LI) structure failed to bind to macrophages, and the disruption of the LI structure by mercaptoethanol led to the failure of binding. The fragments containing the LI structure functioned similarly to ICAM-L, by temporarily retarding host cell growth and cell cycle progression, and inhibiting the Leishmania infection of host macrophages. These results suggest that LI constitutes the main determinant of the ICAM-L molecule in binding to, and infection of, host macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 37, Issues 8–9, July 2007, Pages 1001-1011
نویسندگان
, , , , ,