کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8530843 | 1559727 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of miRNA expression in patients with rheumatoid arthritis during remission and relapse after a 5-year trial of tofacitinib treatment
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The physiopathology of rheumatoid arthritis (RA) is mediated by proinflammatory cytokines, some of which are regulated by the JAK/STAT pathway. Tofacitinib is a JAK inhibitor, but its role in the regulation of microRNAs (miRNAs) is unknown. There is also no information regarding the role of miRNAs in the clinical relapse/remission of RA. The present project aims to identify a signature profile of miRNA expression in a subgroup of RA patients who had to discontinue tofacitinib treatment (because of the ending of a 5-year open-label clinical trial) and to describe the expression of miRNAs during RA remission or flare-up. The relative expression of 61 miRNAs was determined in serum samples with the Firefly⢠BioWorks assay. Statistical analysis was performed by means of Student's t-test and heatmap analysis was performed with Firefly⢠Analysis Workbench software and in the software GraphPad® Prism v5.0. Target prediction and Gene Ontology analysis were carried out using bioinformatic tools. We found a distinctive signature of miRNA expression associated with relapse, featuring upregulated expression of hsaâmiRâ432â5p (pâ¯<â¯0.05). We also found upregulation of hsaâmiRâ194â5p (pâ¯<â¯0.05) in samples of patients with RA flare-up. Gene Ontology analysis of the target genes for hsaâmiRâ432â5p was performed to identify relevant pathways associated with relapse; the implications of these pathways in the physiopathology of RA are discussed. Tofacitinib treatment does not have a direct effect on the expression of measured miRNAs. The changes in hsaâmiRâ432â5p and hsaâmiRâ194â5p are associated with the regulation of proinflammatory pathways and RA flare-up.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 63, October 2018, Pages 35-42
Journal: International Immunopharmacology - Volume 63, October 2018, Pages 35-42
نویسندگان
Julio C. Fernández-Ruiz, Cesar Ramos-Remus, José Sánchez-Corona, José D. Castillo-Ortiz, José J. Castañeda-Sánchez, Yadira Bastian, MarÃa F. Romo-GarcÃa, Fátima Ochoa-González, Adriana E. Monsivais-Urenda, Roberto González-Amaro,