کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8547187 | 1561729 | 2018 | 46 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Putrescine as indicator of manganese neurotoxicity: Dose-response study in human SH-SY5Y cells
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کلمات کلیدی
FDRHMDBm/z - m / zIn vitro toxicity testing - تست سمیت آزمایشگاهیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوRetention time - زمان بازداریMass spectrometry - طیف سنجی جرمیNeurotoxicology - عصب شناسیManganese - منگنز false discovery rate - میزان کشف کاذبhuman metabolome database - پایگاه داده متابولیسم انسانliquid chromatography - کروماتوگرافی مایع
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The present research was to test the hypothesis that Mn causes putrescine accumulation over a physiologically adequate to toxic concentration range in a neuronal cell line. We used human SH-SY5Y neuroblastoma cells treated with MnCl2 under conditions that resulted in cell death or no cell death after 48â¯h. Putrescine and other metabolites were analyzed by liquid chromatography-ultra high-resolution mass spectrometry. Putrescine-related pathway changes were identified with metabolome-wide association study (MWAS). Results show that Mn caused a dose-dependent increase in putrescine over a non-toxic to toxic concentration range. MWAS of putrescine showed positive correlations with the polyamine metabolite N8-acetylspermidine, methionine-related precursors, and arginine-associated urea cycle metabolites, while putrescine was negatively correlated with γ-aminobutyric acid (GABA)-related and succinate-related metabolites (Pâ¯<â¯0.001, FDRâ¯<â¯0.01). These data suggest that measurement of putrescine and correlated metabolites may be useful to study effects of Mn intake in the high adequate to UL range.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 116, Part B, June 2018, Pages 272-280
Journal: Food and Chemical Toxicology - Volume 116, Part B, June 2018, Pages 272-280
نویسندگان
Jolyn Fernandes, Joshua D. Chandler, Ken H. Liu, Karan Uppal, Young-Mi Go, Dean P. Jones,