کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8551146 | 1562107 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The validation & verification of an LC/MS method for the determination of total docosahexaenoic acid concentrations in canine blood serum
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The validation & verification of an LC/MS method for the determination of total docosahexaenoic acid concentrations in canine blood serum The validation & verification of an LC/MS method for the determination of total docosahexaenoic acid concentrations in canine blood serum](/preview/png/8551146.png)
چکیده انگلیسی
Docosahexaenoic acid (DHA), is an omega 3 fatty acid (n-3 FA) that has been shown to play a role in canine growth and physiological integrity and improvements in skin and coat condition. However, potential adverse effects of n-3 FA specifically, impaired cellular immunity has been observed in dogs fed diets with elevated levels of n-3 FA. As such, a safe upper limit (SUL) for total n-3 FAs (DHA and EPA) in dogs has been established. Considering this SUL, sensitive methods detecting DHA in blood serum as a biomarker when conducting n-3 FA supplementation trials involving dogs are required. In this study, an LC-ESI-MS/MS method of DHA detection in dog serum was validated and verified. Recovery of DHA was optimized and parallelism tests were conducted with spiked samples demonstrating that the serum matrix did not interfere with quantitation. The stability of DHA in serum was also investigated, with â80â¯Â°C considered suitable when storing samples for up to six months. The method was linear over a calibration range of 1-500â¯Î¼g/mL and precision and accuracy were found to meet the requirements for validation. This method was verified in an alternative laboratory using a different analytical system and operator, with the results meeting the criteria for verification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 95, June 2018, Pages 198-203
Journal: Regulatory Toxicology and Pharmacology - Volume 95, June 2018, Pages 198-203
نویسندگان
Gerald Patrick Dillon, Jason D. Keegan, Geoff Wallace, Alexandros Yiannikouris, Colm Anthony Moran,