کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553846 | 1562693 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of early and late nuclear DNA damage and extracellular 8-oxodG in normal human skin fibroblasts after carbon ion irradiation compared to X-rays
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کلمات کلیدی
HPLC-ECDSpread-out Bragg peakSOBPRBE8-oxodGOTMSPE8-oxo-7,8-dihydro-2′-deoxyguanosine - 8-اکسو-7،8-دی هیدرو-2'-دگزسی گوانوزینROS - ROSDNA damage - آسیبDNALET - اجازه دهیدSolid-phase extraction - استخراج فاز جامدLinear Energy Transfer - انتقال انرژی خطیOxidative stress - تنش اکسیداتیوOlive tail moment - لحظه دم زیتونSkin - پوستReactive oxygen species - گونههای فعال اکسیژنCarbon ions - یون های کربنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This work aims to evaluate genotoxicity of C-ion vs. X-ray irradiation in normal human skin fibroblasts. Clonogenic cell survival was first evaluated using a linear-quadratic model. Then, early and late genotoxicity was quantified by alkaline comet assay, micronucleus test and 8-oxodG extracellular measurement. Survival strongly decreased after C-ions compared to X-rays with a 4.8-fold decrease at D0 - irradiation dose corresponding to 37% of survival. The level of immediate DNA damage was approximately the same after C-ions or X-rays. However, half-time of DNA repair was 1.3-fold decreased after C-ions compared to X-rays leading to a 2.2-fold increase in remaining damage. In the same way, micronucleus formation was 1.7-fold increased 24â¯h after irradiation and remained 1.8-fold increased 2â¯weeks after C-ions vs. X-rays when secondary oxidative stress wave occurred. This was related to a 2.6-fold increase in binucleated cells percentage in carbon- vs. X-ray-irradiated fibroblasts. Excretion of 8-oxodG was also 1.5-fold increased after C-ions vs. X-rays. 8-oxodG excretion could therefore participate to the appearance of late waves of oxidative stress. These results showed a stronger genotoxicity of C-ions in skin fibroblasts. More investigations are needed to clarify the discrepancy between the two types of radiations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 52, October 2018, Pages 116-121
Journal: Toxicology in Vitro - Volume 52, October 2018, Pages 116-121
نویسندگان
Virginie Prevost, François Sichel, Ivannah Pottier, Alexandre Leduc, Stéphanie Lagadu, Carine Laurent,