کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8554172 | 1562700 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro assessment of skin sensitization, photosensitization and phototoxicity potential of commercial glyphosate-containing formulations
ترجمه فارسی عنوان
ارزیابی آزمایشگاهی در مورد حساسیت پوستی، حساسیت به حساسیت به نور و فوتوتوکسیسیتی ترکیبات تجاری حاوی گلیفواسات
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کلمات کلیدی
مخلوط گیاهان دارویی، گلیفوزات، آزمایش غیر آزمایشی، حساسیت پوستی، حساسیت به پوست پوست، عکاسی زیستی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
This study evaluated the applicability of a modified Direct Peptide Reactivity Assay (DPRA) (OECD N° 442C, 2015) through the 10-fold reduction of reaction volume (micro-DPRA, mDPRA) for skin sensitization evaluation of six commercial glyphosate-containing formulations. In addition, another modification of DPRA was proposed by adding a UVA (5 J/cm2) irradiation step, namely photo-mDPRA, to better characterize (photo)sensitizer materials. The phototoxicity profile of pesticides was also evaluated using the 3T3 Neutral Red Uptake Phototoxicity Test (3T3-NRU-PT) (OECD N° 432, 2004). The mDPRA could represent an environmentally acceptable test approach, since it reduces costs and organic waste. Peptide depletion was greater in photo-mDPRA and changed the reactivity class of each test material, in comparison to mDPRA. Thus, the association of mDPRA with photo-mDPRA was better for correctly characterizing human (photo)sensitizer substances and pesticides. In general, cysteine depletion was greater than that of lysine for all materials tested in both mDPRA and photo-mDPRA. Furthermore, while 3T3-NRU-PT is unable to predict (photo)sensitizers, it was capable of correctly identifying the phototoxic potential of the tested agrochemical formulations. In conclusion, mDPRA plus photo-mDPRA and 3T3-NRU-PT seem to be preliminary non-animal test batteries for skin (photo)sensitization/phototoxicity assessment of chemicals, agrochemical formulations and their ingredients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 45, Part 3, December 2017, Pages 386-392
Journal: Toxicology in Vitro - Volume 45, Part 3, December 2017, Pages 386-392
نویسندگان
Renato Ivan de Ávila, Gabriel Campos Teixeira, Danillo Fabrini Maciel Costa Veloso, Larissa Cleres Moreira, Eliana Martins Lima, Marize Campos Valadares,