کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8630660 | 1568841 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In silico analysis, seasonal variation and gonadotropic regulation of jag1 and its receptor notch1 in testis of spotted snakehead Channa punctatus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Notch1EGFJagged1qPCRTMDORFSSCRT-PCRDSLNICDPest - آفاتSpermatogenesis - اسپرماتوژنزHCG - اچ سی جیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceNotch intracellular domain - دامنه درون سلولی Notchtransmembrane domain - دامنه فرابنفشC-term - دوره CSpermatogonial stem cells - سلول های بنیادی اسپرماتوگونالیepidermal growth factor - عامل رشد اپیدرمیSeasonality - فصلیopen reading frame - قاب خواندن بازC-terminal region - منطقه C ترمینالreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیhuman chorionic gonadotropin - گونادوتروپین کوریونی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study in seasonally breeding spotted snakehead Channa punctatus, for the first time in nonmammalian vertebrates, demonstrated correlation between reproductive phase-dependent testicular expression of ligand Jag1/receptor Notch1 and spermatogenic events. Testicular transcriptome sequencing data from our earlier study in C. punctatus was used in the present study to select the best transcript for jag1 (cpjag1) and notch1 (cpnotch1). The transcripts cpjag1 and cpnotch1 encoded full-length putative proteins of 1215 (cpJag1) and 2475 (cpNotch1) amino acids, respectively. A marked homology in the extracellular domains of Jag1 and Notch1 was observed following their alignment with respective proteins from different vertebrates, suggesting conservation in ligand-receptor interaction in C. punctatus. Both cpJag1 and cpNotch1 showed phylogenetic closeness with their teleostean counterparts, especially with that of Perciformes. Temporal expression of cpjag1 and cpnotch1 in testis depending on reproductive phases showed an appreciably high expression during spermatogenically inactive resting and postspawning phases when seminiferous lobules consisted of spermatogonial stem cells and undifferentiated spermatogonia. Their expression sharply declined during spermatogenically active preparatory and spawning phases. It appears that involvement of cpjag1/cpnotch1 is restricted to inactive phases when spermatogonial stem cells renew themselves and replenish undifferentiated spermatogonia. This assumption is ascertained by an experimental study in which high level of testicular cpjag1/cpnotch1 expression in control fish of resting phase markedly decreased after administration of human chorionic gonadotropin that is known to induce proliferation and differentiation of spermatogonia and spawning of spermatozoa.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 266, 15 September 2018, Pages 166-177
Journal: General and Comparative Endocrinology - Volume 266, 15 September 2018, Pages 166-177
نویسندگان
Alivia Roy, Reetuparna Basak, Umesh Rai,