کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8634422 | 1569099 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pelargonidin-3-O-glucoside and its metabolites have modest anti-inflammatory effects in human whole blood cultures
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کلمات کلیدی
LPSPGAFCS4-HBAPCA - PCAAnthocyanin - آنتوسیانین4-Hydroxybenzoic acid - اسید 4-هیدروکسی بنزوئیکprotocatechuic acid - اسید پروتکتاتوکوئیکinflammation - التهاب( توروم) interleukin - اینترلوکینStrawberry - توت فرنگیtumor necrosis factor-α - تومور نکروز عامل αfetal calf serum - سرم گوساله جنینTNF-α - فاکتور نکروز توموری آلفاPhagocytosis - فاگوسیتوز یا بیگانهخواری Primary cell culture - فرهنگ سلولی اولیهlipopolysaccharide - لیپوپلی ساکارید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pelargonidin-3-O-glucoside and its metabolites have modest anti-inflammatory effects in human whole blood cultures Pelargonidin-3-O-glucoside and its metabolites have modest anti-inflammatory effects in human whole blood cultures](/preview/png/8634422.png)
چکیده انگلیسی
This study hypothesized that the predominant strawberry anthocyanin, pelargonidin-3-O-glucoside (Pg-3-glc), and 3 of its plasma metabolites (4-hydroxybenzoic acid, protocatechuic acid, and phloroglucinaldehyde [PGA]) would affect phagocytosis, oxidative burst, and the production of selected pro- and anti-inflammatory cytokines in a whole blood culture model. For the assessment of phagocytosis and oxidative burst activity of monocytes and neutrophils, whole blood was preincubated in the presence or absence of the test compounds at concentrations up to 5 μmol/L, followed by analysis of phagocytic and oxidative burst activity using commercially available test kits. For the cytokine analysis, diluted whole blood was stimulated with lipopolysaccharide in the presence or absence of the test compounds at concentrations up to 5 μmol/L. Concentrations of selected cytokines (tumor necrosis factor-α, interleukin [IL]-1β, IL-6, IL-8, and IL-10) were determined using a cytometric bead array kit. There were no effects of any of the test compounds on phagocytosis of opsonized or nonopsonized Escherichia coli or on oxidative burst activity. Pg-3-glc and PGA at 0.08 μmol/L increased the concentration of IL-10 (P < .01 and P < .001, respectively), but there was no effect on tumor necrosis factor-α, IL-1β, IL-6, and IL-8, and there were no effects of the other compounds. In conclusion, this study demonstrated a lack of effect of these compounds on the opsonization, engulfment, and subsequent destruction of bacteria. Pg-3-glc and PGA, at physiologically relevant concentrations, had anti-inflammatory properties; however, effects were modest, only observed at the lowest dose tested and limited to IL-10.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 46, October 2017, Pages 88-95
Journal: Nutrition Research - Volume 46, October 2017, Pages 88-95
نویسندگان
Anna M. Amini, Karolin Muzs, Jeremy PE. Spencer, Parveen Yaqoob,