کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644372 | 1569758 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of the TaWTG1 in bread wheat (Triticum aestivum L.)
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کلمات کلیدی
RPKMQTLTFBSTPMESTs - EST هاStress - استرس یا فشار روانیExpression pattern - الگوی بیانreads per kilobase per million reads - به ازای هر کیلو بایت در هر کیلو بایت خوانده می شودComparative analysis - تجزیه و تحلیل تطبیقیQuantitative trait loci - تعداد صفات صفاتcoding sequences - توالی کدگذاریexpression sequence tags - تگ های توالی بیانtranscripts per million - رونویسی در هر میلیونStructure - ساختار یا سازهTranscription factor binding sites - سایت های مرتبط با عامل رونویسیTranscription factor - عامل رونویسیCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
OsWTG1 (LOC_Os08g42540.1) functions as an important factor determining grain size and shape in rice. Our understanding on its ortholog in wheat, TaWTG1, is limited. Here, we identified and mapped TaWTG1 in wheat, characterized its gene and protein structures, predicted transcription factor binding sites of its promoter, and the expression patterns was also analysed bases on real-time quantitative PCR and public available microarray data. The WTG1 orthologs in barley (HvWTG1), rice (OsWTG1), Aegilops tauschii (AtWTG1), Triticum urartu (TuWTG1), Triticum turgidum (TtWTG1) and Brachypodium distachyon (BdWTG1) were also identified for comparative analyses. TaWTG1 was mapped onto the short arms of group 7 chromosomes (7AS, 7BS, and 7DS). Multiple alignments indicated that WTG1 possesses eight exons and seven introns in all of the orthologs, except for the orthologs on 7A of wild emmer and on 7D of A. tauschii (seven exons and six introns). An exon-intron junction composed of intron 2 to intron 3 and exon 2 to exon 4 was highly conserved. The protein of WTG1 exists a conserved domain (Peptidase_C65). WTG1 was mainly expressed in wheat roots, spikes and grains, in barley caryopsis and roots, and in rice anthers. Drought and heat stresses significantly regulated the expression of TaWTG1 in wheat. In barley, WTG1 was significantly down-regulated under Fusarium at late stage. In addition, significant correlations between the expression patterns of predicted transcription factors and WTG1 were also detected. Overall, the results presented here broaden our knowledge on WTG1 and will be helpful for its manipulation aiming at dissecting its function in plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 678, 15 December 2018, Pages 23-32
Journal: Gene - Volume 678, 15 December 2018, Pages 23-32
نویسندگان
Han Zhang, Jian Ma, Jiajun Liu, Yang Mu, Huaping Tang, Yaxi Liu, Guangdeng Chen, Qiantao Jiang, Guoyue Chen, Yuming Wei, Youliang Zheng, Xiujin Lan,