Anti-inflammatory effect of Calycosin glycoside on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells

Objective to find the Calycosin glycoside (CG) potential anti-inflammatory effect. The anti-inflammatory activity of CG was evaluated by lipopolysaccharide (LPS)-induced RAW 264.7 cells, and the IL-6, IL-1β, TNF-α and PGE2 level were measured by ELISA; NO levels in the culture media were determined using the Griess reaction. The iNOS and COX-2 mRNA expressions were measured by qRT-PCR and the phosphorylation of IκBα, p65, ERK, JNK, and p38 was determined by western blotting. The CG could markedly inhibit the productions of NO and PGE2 and markedly inhibit the productions of TNF-α, IL-1β and IL-6. CG could suppress mRNA expression of iNOS, COX-2 and protein phosphorylation of IκBα, p65, ERK, JNK, and p38 in LPS-induced RAW 264.7 cells. These findings indicate that CG exhibited potent anti-inflammatory activity through the NF-κB and MAPK signal pathway, and as a potential therapeutic agent against inflammatory diseases.