کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645391 | 1569784 | 2018 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The expression pattern and potential functions of PHB in the spermiogenesis of Phascolosoma esculenta
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کلمات کلیدی
Phascolosoma esculentaPBSqPCRFITCPHBORFopa1 - grandpa1ROS - ROSoptic atrophy 1 - آتروفی اپتیکی 1Spermiogenesis - اسپرمیوژنزimm - انحصارImmunofluorescence - ایمونوفلورسانسinner mitochondrial membrane - درونی غشای میتوکندریreal-time quantitative PCR - زمان واقعی PCR کمیelectron transfer chain - زنجیره انتقال الکترونPhosphate buffered saline - فسفات بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتfluorescence in situ hybridization - فلورسانس در هیبریداسیون در محلopen reading frame - قاب خواندن بازFish - ماهیProhibitin - ممنوعیتMitochondrion - میتوکندریونhematoxylin–eosin - هماتوکسیلین ائوزینETc - و غیرهReactive oxygen species - گونههای فعال اکسیژنubiquitination - یوبی کوئیتینه شدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prohibitin (PHB) is a ubiquitous, evolutionarily conserved protein that is mainly localized in the inner mitochondrial membrane and exerts various mitochondrial functions. Here, we first cloned the phb gene from P. esculenta. The Pe-PHB protein has high homology and a similar protein structure to that of other animals, and it can be divided into the N-terminal hydrophobic/transmembrane domain, SPFH domain, and C-terminal coiled-coil domain. The Pe-phb gene is widely expressed, and the gene expression of phb is highest in coelomic fluid where spermiogenesis occurs, indicating a specific function in the coelom. We further observed continuous expression of the phb gene and localization of PHB proteins in mitochondria during spermiogenesis, indicating that PHB, as a mitochondrial component, may play a role during this process via its mitochondrial function. In addition, ubiquitination of mitochondria was detected, and the PHB signal was co-localized with the poly-ubiquitin signal during spermiogenesis. Mature sperm also showed ubiquitination of mitochondria and PHB. Therefore, PHB may be a substrate of poly-ubiquitin to regulate the ubiquitination of mitochondria and even subsequent elimination during P. esculenta spermiogenesis, and it has a potential role in guaranteeing the maternal inheritance of mitochondria. Taken together, these results support the hypothesis that PHB participates in the spermiogenesis of P. esculenta by maintaining the normal function of mitochondria and regulating the degradation of mitochondria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 652, 30 April 2018, Pages 25-38
Journal: Gene - Volume 652, 30 April 2018, Pages 25-38
نویسندگان
Cong-Cong Hou, Xin-Ming Gao, Jie Ni, Dan-Li Mu, Hai-Yan Yang, Cheng Liu, Jun-Quan Zhu,