کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645775 | 1569793 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phylogeny and expression patterns of two apolipoprotein E genes in the flatfish Senegalese sole
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
BCIPYSLmAbHDLNeuromastsTLBVLDLSyntenyNBTABCA1DPHPFADMSO - DMSOWISH - آرزو کردنapolipoprotein E - آپولیپوپروتئین EGene expression - بیان ژنnitro blue tetrazolium - تترازولیم نیترو آبیLarval feeding - تغذیه لاروTail bud - جوانه دمTaste buds - جوانه طعمSenegalese sole - خورشید سنگالCNS - دستگاه عصبی مرکزیMouth - دهانIntestine - رودهdays post-hatch - روز بعد از اخراجcentral nervous system - سیستم عصبی مرکزیPharynx - فریونکسHeart - قلب Yolk syncytial layer - لایه یون سیکسیتی یولکLipoprotein - لیپوپروتئینvery low density lipoprotein - لیپوپروتئین چگالی بسیار کم استLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)High-density lipoproteins - لیپوپروتئینهای با چگالی بالاBrain - مغزHindbrain - هیدروپینOtic vesicle - ویسکوزیتهparaformaldehyde - پارافرمالدهیدSkin - پوستEye - چشمLiver - کبد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phylogeny and expression patterns of two apolipoprotein E genes in the flatfish Senegalese sole Phylogeny and expression patterns of two apolipoprotein E genes in the flatfish Senegalese sole](/preview/png/8645775.png)
چکیده انگلیسی
The apolipoprotein E (ApoE) is a key component of several lipoproteins involved in lipid homeostasis. In this study, two cDNA sequences encoding ApoE (referred to as apoEa and apoEb) were characterized in the flatfish Solea senegalensis. The predicted peptides contained conserved structural blocks related with their capacity for lipid binding and lipoprotein receptor interaction. At genomic level, both genes contained five exons and four introns and they were organized into two tandem arrays with apoA-IV gene copies. The phylogenetic analysis clearly separated them into two well-supported clusters that matched with their organization in the genome of teleosts. Whole-mount in situ hybridization located the apoEa signal in the yolk syncytial layer (YSL) of lecitothrophic larval stages (0Â dph) and in the anterior intestine of exotrophic larvae and benthic fish. In the case of apoEb, hybridization signals were located in the YSL, tail bud, eyes and mouth at 0Â dph and in the otic vesicle, hindbrain, eyes, pharynx, mouth, heart and intestine at 1Â dph. In exotrophic larvae, apoEb was ubiquitously expressed in several tissues such as taste buds, brain, mouth, nostril, gills, intestine, liver and around the neuromasts and eyes. Quantification of mRNA levels in pools of whole larvae confirmed distinct expression patterns with a significant reduction of apoEa and an increase of apoEb mRNA levels throughout larval development. Moreover, only apoEa transcripts increased in response to food supply suggesting that this paralog mostly participates in the absorption and transport of dietary lipids and the apoEb in the redistribution of endogenous lipids as well as in neural tissue regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 643, 15 February 2018, Pages 7-16
Journal: Gene - Volume 643, 15 February 2018, Pages 7-16
نویسندگان
Javier Roman-Padilla, Ana RodrÃguez-Rúa, Carlos Carballo, Manuel Manchado, Ismael Hachero-Cruzado,