کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866256 | 1470949 | 2016 | 6 صفحه PDF | دانلود رایگان |

• We developed a NECL aptasensor for thrombin detection
• ECL resonance energy transfer was used here.
• The aptasensor showed a low detection limit of 31 aM.
• The aptasensor could be applied to detect thrombin in human serum.
Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultrasensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS coresmall/shellthick quantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was “turned on” because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N=3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples.
Journal: Biosensors and Bioelectronics - Volume 82, 15 August 2016, Pages 26–31