کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866591 1470980 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A portable chemical sensor for histidine based on the strategy of click chemistry
ترجمه فارسی عنوان
حسگر شیمیایی قابل حمل برای هیستیدین بر اساس استراتژی شیمی کلیک کنید
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A portable chemical sensor for histidine was proposed.
• The sensor combines the characters of personal glucose meters and click chemistry.
• The detection limit is 3.4 nmol/L and the linear range is 0.01~100 μM.
• The sensor has been successfully applied to detect histidine in milk.

A novel portable chemical sensor is developed in combination of the personal glucose meters (PGM) with click chemistry for sensitive and selective determination of histidine. Invertase-labeled alkynyl-DNA can be modified onto the surfaces of Streptavidin Magnespheres Paramagnetic Particles (PMPs) through copper(I) catalyzed azide–alkyne cycloaddition (CuAAC) reaction and formed invertase-functionalized PMPs, which can be separated easily. The presence of invertase can convert sucrose to glucose and can be monitored by the PGM easily. The presence of histidine can inhibit the CuAAC, so the read-out signal of PGM decreased. The difference in signals from the PGM before and after addition of histidine has a good linear correlation with the logarithm of the histidine concentrations in the range of 0.01~100 μM with a detection limit of 3.4 nM, which is lower than those of many other chemical sensors. Moreover, the assay of histidine in milk samples is demonstrated with satisfactory results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 51, 15 January 2014, Pages 386–390
نویسندگان
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