کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866709 1470978 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An automatic enzyme immunoassay based on a chemiluminescent lateral flow immunosensor
ترجمه فارسی عنوان
آنزیم اتوماتیک آنالیز ایمنی بر اساس یک ایمونوسنسور جریان جانبی شیمیایی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We developed novel Lateral flow immunosensor based enzyme immunoassay strip sensor.
• The key concept relies on a delayed-release effect of chemiluminescence substrates by an asymmetric polysulfone membrane (ASPM).
• The multiple steps of EIA reactions are automatically implemented on lateral flow immunosensor.
• Implementation of the sensor was validated by measuring the high sensitivity C-reactive protein (hs-CRP) level in human serum.

Microfluidic integrated enzyme immunosorbent assay (EIA) sensors are efficient systems for point-of-care testing (POCT). However, such systems are not only relatively expensive but also require a complicated manufacturing process. Therefore, additional fluidic control systems are required for the implementation of EIAs in a lateral flow immunosensor (LFI) strip sensor. In this study, we describe a novel LFI for EIA, the use of which does not require additional steps such as mechanical fluidic control, washing, or injecting. The key concept relies on a delayed-release effect of chemiluminescence substrates (luminol enhancer and hydrogen peroxide generator) by an asymmetric polysulfone membrane (ASPM). When the ASPM was placed between the nitrocellulose (NC) membrane and the substrate pad, substrates encapsulated in the substrate pad were released after 5.3±0.3 min. Using this delayed-release effect, we designed and implemented the chemiluminescent LFI-based automatic EIA system, which sequentially performed the immunoreaction, pH change, substrate release, hydrogen peroxide generation, and chemiluminescent reaction with only 1 sample injection. In a model study, implementation of the sensor was validated by measuring the high sensitivity C-reactive protein (hs-CRP) level in human serum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 53, 15 March 2014, Pages 330–335
نویسندگان
, , ,