Recent advances in olfactory receptor-basedbiosensors

The biological olfactory system can recognize and discriminate thousands of volatile organic compounds (VOCs) with extremely high sensitivity and specificity. The most fundamental elements are olfactory receptors (ORs) in the cilia of olfactory sensory neurons (OSNs), which contribute greatly to the high-performance olfactory system. The excellent properties of ORs are generally recognized in the development of biomimetic OR-based biosensors. Over the past two decades, much work has been done in developing OR-based biosensors due to their promising potential in many applications. In this article, we will outline the latest advances of OR-based biosensors. Two current crucial issues in this field will be discussed, namely, the production methods and immobilization techniques of ORs. We will also elaborate on various OR-based biosensors and their latest developments. Finally, current research trends and future challenges in this field will be discussed.

► This review paper introduces the history of olfactory receptor-based biosensors comprehensively.
► Two hot issues in the development of olfactory receptor-based biosensors are discussed in detail.
► The latest developments of various olfactory receptor-based biosensors are presented.
► The future research trends are pointedout.