کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867123 | 1470989 | 2013 | 6 صفحه PDF | دانلود رایگان |

A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of a layer of biorecognition molecules. The sensor surface has been shown to maintain its performance after freeze-drying providing a long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology are demonstrated using three antigen–antibodypairs.
► An integrated solution of a novel robust and end-user ready biosensor method using ellipsometric detection is shown.
► We developed linker-free protein covalent binding surfaces for the biosensors.
► Optimized optical design of the sensors is demonstrated.
► Freeze-dry method was used to the biosensors prior to storage at ambient temperature.
► merits of the methodology were demonstrated using three antigen–antibodypairs.
Journal: Biosensors and Bioelectronics - Volume 42, 15 April 2013, Pages 447–452