کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867215 909779 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunosensor based on magnetic relaxation switch and biotin–streptavidin system for the detection of Kanamycin in milk
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Immunosensor based on magnetic relaxation switch and biotin–streptavidin system for the detection of Kanamycin in milk
چکیده انگلیسی

A rapid, sensitive, and simple immunosensor was developed for the detection of Kanamycin (KM) in milk. This immunosensor is based on magnetic relaxation switch (MRS) assay and biotin–streptavidin system (B–SA system). The target analyte (KM) competed with those on the surface of the superparamagnetic iron oxide (SPIO) nanoparticles and hence affected the formation of SPIO aggregates. The dispersed and aggregated states of SPIO can modulate the spin–spin relaxation time (T2) of the neighboring water molecule. T2 was then changed as an effect of the target analyte. The B–SA system was used to amplify the SPIO binding, thus enhance the sensitivity. The detection working was 1.5 to 25.2 ng mL−1 and limit of detection (LOD) was determined to be 0.1 ng mL−1. The LOD of the immunosensor decreased tenfold, and its analysis time (45 min) was much shorter than that of enzyme-linked immunosorbent assay (6 h to 8 h). The average recoveries of the KM at various spiking levels ranged from 80.2% to 85.6% with a relative standard deviation (RSD) below 4.0%. The results showed that the MRS immunosensor was a promising platform for the determination of small molecular residues because of its high sensitivity, specificity, homogeneity, and speed.


► A magnetic relaxation switch immunosensor was developed for the detection of Kanamycin.
► The LOD of this immunosensor decreased ten fold compared to that of ELISA.
► The analysis time of immunosensor (45 min) was much shorter compared with that of ELISA.
► The biotin–streptavidin system increased the sensitivity and the accuracy of this immunosensor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 39, Issue 1, 15 January 2013, Pages 112–117
نویسندگان
, , , , , , , , ,