Detection of multi-color fluorescent objects with single photon spectrometer

Single photon counting is the most sensitive and accurate method for detection of very weak fluorescent signals obtained in many applications such as DNA sequencing, detection of biological reporters on micro-beads, detection of droplets in micro-fluidic systems, etc. In this paper we describe the use of single photon spectrometer for detection and characterization of very weak multicolor fluorescence produced by mixtures of various fluorescent dyes and quantum dots.

► Universal fiberized single photon spectrometer based on 32-channel PMT sensor.
► Method for accurate determination of composition of quantum dots mixtures.
► Model for computer simulation of measurements of dye mixtures.
► Accurate measurements of color-encoded microbeads.