کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867335 909781 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulating electron transfer properties of gold nanoparticles for efficient biosensing
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modulating electron transfer properties of gold nanoparticles for efficient biosensing
چکیده انگلیسی

Present study concerns modulating the electron transfer properties of gold nanoparticles through amino acid induced coupling among them. In addition to conductivity, the amino functionalization of the nanoparticles results in enhanced activity and operational stability of the biosensor fabricated using the same. Nanoparticles synthesized using amino acid as reducing agent (average diameter—20 nm), incorporate the natural coupling property of amino acids and are seen to align in a chain-like arrangement. The coupling of the individual nanoparticles to form chain like structure was confirmed by both absorption spectroscopy as well as transmission electron microscopy. The glucose biosensor developed by adsorption of glucose oxidase (GOx) enzyme onto these coupled gold nanoparticles showed enhanced efficiency as compared to the one with glucose oxidase immobilized onto gold nanoparticles synthesized using the conventional method (trisodium citrate as reducing agent). The fabricated biosensor demonstrated a wide linear concentration range from 1 μM–5 mM and a high sensitivity of 47.2 μA mM−1 cm−2. Also, an enhanced selectivity to glucose was observed with negligible interference in the physiological range, from easily oxidizable biospecies, e.g. uric acid and ascorbic acid. Furthermore, the electrochemical biosensor has excellent long term stability- retaining greater than 85% of the biosensor activity up to 60 days.


► Enhanced electron transfer properties of coupled gold nanoparticles in comparison to spherical gold nanoparticles.
► Improved electron shuttling between enzyme catalytic center and electrode surface.
► Amino acids induced covalently coupled gold nanoparticles.
► Operationally stable biosensor with 15% reduction in activity after 60 days.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 37, Issue 1, August–September 2012, Pages 30–37
نویسندگان
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