کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867571 909785 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A biosensor for cholesterol based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A biosensor for cholesterol based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence
چکیده انگلیسی

A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase (ChOx) was adsorbed on the surface of AuNPs to construct a cholesterol biosensor. The stepwise fabrication processes were characterized with cyclic voltammetry and atomic force microscopy. The ECL behaviors of the biosensor were also investigated. It was found that AuNPs not only provided larger surface area for higher ChOx loading but also formed the nano-structured interface on the electrode surface to improve the analytical performance of the ECL biosensor for cholesterol. Besides, based on the efficient catalytic ability of AuNPs to luminol ECL, the response of the biosensor to cholesterol was linear range from 3.3 μM to 1.0 mM with a detection limit of 1.1 μM (S/N = 3). In addition, the prepared ECL biosensor exhibited satisfying reproducibility, stability and selectivity. Taking into account the advantages of ECL, we confidently expect that ECL would have potential applications in biotechnology and clinical diagnosis.


► A biosensor for cholesterol based on AuNPs-catalyzed luminol–H2O2 system electrogenerated chemiluminescence.
► Combine with the high sensitivity of catalytic properties of cholesterol oxidase and AuNPs.
► l-cys-rGO composites possessed larger surface areas and more active sites to immobilize large amounts of AuNPs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 32, Issue 1, 15 February 2012, Pages 288–292
نویسندگان
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