کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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867714 | 909791 | 2011 | 7 صفحه PDF | دانلود رایگان |
We report a sensitive method for visual detection of mercury ions (II) (Hg2+) in aqueous solution by using gold nanoparticles (Au-NPs) and thymine (T)-rich hairpin DNA probes. The thiolated hairpin DNA probe was immobilized on the Au-NP surface through a self-assembling method. Another thymine-rich, digoxin-labeled DNA probe was introduced to form DNA duplexes on the Au-NP surface with thymine–Hg2+–thymine (T–Hg2+–T) coordination in the presence of Hg2+. The Au-NPs associated with the formed duplexes were captured on the test zone of a lateral flow strip biocomponent (LFSB) by immunoreaction events between the digoxin on the duplexes and anti-digoxin antibodies on the LFSB. The accumulation of Au-NPs produced a characteristic red band on the test zone, enabling visual detection of Hg2+ without instrumentation. A detection limit of 0.1 nM was obtained under optimal experimental conditions. This method provides a simple, rapid, sensitive approach for the detection of Hg2+ and shows great promise for point-of-care and in-field detection of environmentally toxic mercury.
Journal: Biosensors and Bioelectronics - Volume 26, Issue 11, 15 July 2011, Pages 4464–4470