کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867720 | 909791 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Reversible thrombin detection by aptamer functionalized STING sensors Reversible thrombin detection by aptamer functionalized STING sensors](/preview/png/867720.png)
Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution.
Journal: Biosensors and Bioelectronics - Volume 26, Issue 11, 15 July 2011, Pages 4503–4507