کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867980 | 909799 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A cyclodextrin host–guest recognition approach to an electrochemical sensor for simultaneous quantification of serotonin and dopamine A cyclodextrin host–guest recognition approach to an electrochemical sensor for simultaneous quantification of serotonin and dopamine](/preview/png/867980.png)
An electrochemical sensor for simultaneous quantification of serotonin (5-hydroxytryptamine, 5-HT) and dopamine (DA) using a β-cyclodextrin/poly(N-acetylaniline)/carbon nanotube composite modified carbon paste electrode has been developed. Synergistic effect of multi-walled carbon nanotube (MWCNT) in addition to the pre-concentrating effect of β-cyclodextrin (β-CD) as well as its different inclusion complex stability with 5-HT and DA was used to construct an electrochemical sensor for quantification of these important neurotransmitters. The overlapping anodic peaks of 5-HT and DA at 428 mV on bare electrode resolved in two well-defined voltammetric peaks at 202 and 363 mV vs. Ag/AgCl respectively. The oxidation mechanism of 5-HT and DA on the surface of the electrode was investigated by cyclic voltammetry and it was found that the electrode processes are pH dependent and electrochemical oxidation of 5-HT is totally irreversible while the electrode gave a more reversible process to DA.Under optimized conditions, linear calibration curves were obtained in the range of about 4–200 μM with a detection limits down to sub-μM levels (S/N = 3) after 20-s accumulation, for both. The proposed sensor was shown to be remarkably selective for 5-HT and DA in matrices containing different species including ascorbic acid and uric acid. The suitability of the developed method was tested for the determination of 5-HT and DA in the Randox Synthetic Plasma samples and acceptable recoveries were obtained for a set of spiked samples.
► DA and 5-HT electrochemical biosensor based on a cyclodextrin host-guest recognition approach.
► Cyclodextrin accumulate DA and 5-HT at electrode surface and CNT catalyze their oxidation.
► In addition, different inclusion complex stability of cyclodextrin with DA and 5-HT resolved their overlapping oxidation potential.
► Linear calibration curves were obtained in the range of about 4–200 μM for both.
Journal: Biosensors and Bioelectronics - Volume 26, Issue 12, 15 August 2011, Pages 4674–4680