کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868051 909801 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Conductive carbon nanoparticles-based electrochemical immunosensor with enhanced sensitivity for α-fetoprotein using irregular-shaped gold nanoparticles-labeled enzyme-linked antibodies as signal improvement
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Conductive carbon nanoparticles-based electrochemical immunosensor with enhanced sensitivity for α-fetoprotein using irregular-shaped gold nanoparticles-labeled enzyme-linked antibodies as signal improvement
چکیده انگلیسی

A new electrochemical immunoassay protocol for sensitive detection of α-fetoprotein (AFP, as a model) is designed using carbon nanoparticles (CNPs)-functionalized biomimetic interface as immunosensing probe and irregular-shaped gold nanoparticles (ISNGs)-labeled horseradish peroxidase-anti-AFP conjugates (HRP-anti-AFP-ISNG) as trace label. The low-toxic and high-conductive CNPs provided a high capacity nanoparticulate immobilization surface and a facile pathway for electron transfer. In comparison with conventional label methods, i.e. spherical gold nanoparticles-labeled HRP-anti-AFP and HRP-labeled anti-AFP, the electrochemical immunosensor using HRP-anti-AFP-ISNGs as trace labels exhibited high bioelectrocatalytic response toward enzyme substrate and a wide dynamic range from 0.02 to 4.0 ng/mL with a low detection limit of 10 pg/mL toward AFP (at 3σ). The developed immunoassay method showed good selectivity and acceptable reproducibility. Clinical serum samples with various AFP concentrations were evaluated by using the electrochemical immunosensor and the referenced enzyme-linked immunosorbent assay (ELISA), respectively, and received in good accordance with results obtained from these two methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 12, 15 August 2010, Pages 2657–2662
نویسندگان
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