کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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868136 | 909803 | 2011 | 7 صفحه PDF | دانلود رایگان |
This paper reports an enrichment platform for botulinum neurotoxin type B (BoNT/B) that has been realized through the fusion of bioconjugation chemistry and microfluidics. Micrometer-sized magnetic beads were conjugated to a 22mer synthetic peptide derived from the synaptotagmin II (Syt II) neuronal protein that is specific for BoNT/B binding. Exposure to BoNT/B in buffer, whole milk and fruit juices resulted in toxin capture, which was confirmed using immunofluorescence. Peptide-modified beads were integrated into arrayed, polymeric microfluidic channels, and all assay steps, from capture to detection, were performed directly in the microchannels, thereby simplifying assay utility and increasing throughput relative to existing detection methodologies. Our sensitive microscale approach required only 7 μL of intentionally adulterated sample without any pre-processing (i.e. dilution, centrifugation, filtering), and with a “hands-on” time of only 1 h to detect 16.6 pg of BoNT/B in whole milk.
Journal: Biosensors and Bioelectronics - Volume 26, Issue 5, 15 January 2011, Pages 1929–1935