کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868268 | 909805 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe Development of a method to evaluate caspase-3 activity in a single cell using a nanoneedle and a fluorescent probe](/preview/png/868268.png)
A method to detect an enzymatic reaction in a single living cell using an atomic force microscope equipped with an ultra-thin needle (a nanoneedle) and a fluorescent probe molecule was developed. The nanoneedle enables the low-invasive delivery of molecules attached onto its surface directly into a single cell. We hypothesized that an enzymatic reaction in a cell could be profiled by monitoring a probe immobilized on a nanoneedle introduced into the cell. In this study, a new probe substrate (NHGcas546) for caspase-3 activity based on fluorescent resonance energy transfer (FRET) was constructed and fixed on a nanoneedle. The NHGcas546-modified nanoneedle was inserted into apoptotic cells, in which caspase-3 is activated after apoptosis induction, and a change in the emission spectrum of the immobilized probe could be observed on the surface of the nanoneedle. Thus, we have developed a successful practical method for detecting a biological phenomenon in a single cell. We call the method MOlecular MEter with Nanoneedle Technology (MOMENT).
Journal: Biosensors and Bioelectronics - Volume 25, Issue 1, 15 September 2009, Pages 22–27