کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868303 909805 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance
چکیده انگلیسی

A stable and sensitive toxin residues immunosensor based on the relaxation of magnetic nanoparticles was developed. The method was performed in one reaction and offered sensitive, fast detection of target toxin residues in water. The target analyte, microcystin-LR (MC-LR) in Tai lake water, competed with the antigens on the surface of the magnetic nanoparticles and then influenced the formation of aggregates of the magnetic nanoparticles. Accordingly, the magnetic relaxation time of the magnetic nanoparticles was changed under the effect of the target analyte. The calibration curve was deduced at different concentrations of the target analyte. The limit of detection (LOD) of MC-LR was 0.6 ng g−1 and the detection range was 1–18 ng g−1. Another important feature of the developed method was the easy operation: only two steps were needed (1) to mix the magnetic nanoparticle solution with the sample solution and (2) read the results through the instrument. Therefore, the developed method may be a useful tool for toxin residues sensing and may find widespread applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 1, 15 September 2009, Pages 240–243
نویسندگان
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