Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)32+ doped silica nanoparticles

An ethanol biosensor, based on the electrogenerated chemiluminescence of Ru(bpy)32+-doped silica nanoparticles (RuSiNPs), was investigated in this study. The biosensor was a modified glassy carbon electrode, where alcohol dehydrogenase was crosslinked to RuSiNPs, and then immobilized on the electrode surface using chitosan. The results indicated that the biosensor exhibited excellent performance during ethanol determination with a wide linear range (10−7 to 10−2 M), low detection limit (5.0 × 10−8 M) and good stability.