کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868313 909806 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a disposable amperometric biosensor for salicylate based on a plastic electrochemical microcell
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of a disposable amperometric biosensor for salicylate based on a plastic electrochemical microcell
چکیده انگلیسی

The use of an amperometric biosensor for rapid salicylate determination in blood is described. Photolitography was used to make gold electrodes on a polyester film. The plastic microcell was characterized using cyclic voltammetry to demonstrate the electrochemical performance of the system. The biosensor was constructed by immobilizing salicylate hydroxylase onto the working electrode of the plastic electrochemical microcell. The optimized working conditions were 0.1 mol L−1 phosphate buffer at pH 7.6 with 0.5 mmol L−1 of NADH and 300 mV vs. Au as the applied potential. The resulting biosensor exhibited a high sensitivity (97.4 nA/mmol L−1 salicylate) and an adequate linear response range (1.2 × 10−4 to 1.0 × 10−3 mol −1). The biosensor performance was verified by determining salicylate in spiked blood samples and the results were statistically equivalent to the values obtained from the standard Trinder spectrophotometric method, with a 95% confidence level. This study shows the potential development of a portable, inexpensive and disposable device for point-of-care monitoring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 10, 15 June 2010, Pages 2200–2204
نویسندگان
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