کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868698 909812 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface plasmon resonance immunosensor for early diagnosis of Asian rust on soybean leaves
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Surface plasmon resonance immunosensor for early diagnosis of Asian rust on soybean leaves
چکیده انگلیسی

Soybean rust (Asian rust) is a disease that occurs in soy cultures, negatively affecting pod formation and final grain weight and reducing value and product quality. Early identification of fungus in the plants prevents severe farming losses and spread to neighboring cultures. In this paper, a fast response sensor was developed based on surface plasmon resonance to detect Asian rust in soybean leaf extract at early stages of the disease. The antibody anti-Phakopsora pachyrhizi (pathogen) was covalently immobilized on a gold substrate via a self-assembled monolayer (SAM) of thiols using cysteamine-coupling chemistry. This immunosensor presented a linear response range for the antigen from 3.5 to 28.0 μg mL−1 (r2 = 0.996). The effects of the antibody amount and the surface blocking to minimize non-specific adsorption on immunosensor response were evaluated. These studies provide new perspectives on using SPR technology for the development of a highly sensitive sensor for agricultural applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2483–2487
نویسندگان
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