کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868699 | 909812 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNA detection on ultrahigh-density optical fiber-based nanoarrays DNA detection on ultrahigh-density optical fiber-based nanoarrays](/preview/png/868699.png)
Nanoarrays for DNA detection were fabricated on etched nanofiber bundles based on recently developed techniques for microscale arrays. Two different-sized nanoarrays were created: one with 700 nm feature sizes and a 1 μm center-to-center pitch (∼1 × 106 array elements/mm2) and one with 300 nm feature sizes and a 500 nm center-to-center pitch (4.6 × 106 array elements/mm2). A random, multiplexed array composed of oligonucleotide-functionalized nanospheres was constructed and used for parallel detection and analysis of fluorescently labeled DNA targets. We have used these arrays to detect a variety of target sequences including Bacillus thuringiensis kurstaki and vaccina virus sequences, two potential biowarfare agents, as well as interleukin-2 sequences, an immune system modulator that has been used for the diagnosis of HIV.
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2488–2493