کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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868807 | 909814 | 2010 | 7 صفحه PDF | دانلود رایگان |
A novel type of colloidal carbon sphere array (CSA) was developed for the fabrication of disposable electrochemical immunosensor. The CSA was successfully prepared on indium tin oxide (ITO) substrate in a simple manner and the scanning electron micrograph confirmed that a single-layered arrangement of the carbon spheres with its (1 1 1) plane paralleled the substrate's surface. The CSA modified electrode has a higher surface area and exhibits a more sensitive electrochemical response than a normal carbon-based electrode with the same geometric area. An Immunoglobin A (IgA) immunosensor was constructed by the covalent bonding of IgA antibody molecules with the CSA aided by large numbers of carboxyl groups on the surface of carbon spheres. The immunosensor exhibited a wide linear response to IgA ranging from 0.1 to 200 ng mL−1 by electrochemical impedance spectroscopy (EIS) technique. The detection of IgA levels in three sera obtained from hospital samples showed acceptable accuracy.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 5, 15 January 2010, Pages 1130–1136