کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868812 | 909814 | 2010 | 6 صفحه PDF | دانلود رایگان |
An amperometric biosensor for the detection of the reduced nicotinamide cofactors NADH and NADPH was designed, based on the electrochemical oxidation of NAD(P)H with an iron oxide/carbon black composite (Fe2O3/CB) electrode. The electrode exhibited excellent performances in that it led to a substantial decrease in the overpotential of electrochemical NADH oxidation. Iron oxide plays a significant role as a catalyst for NADH oxidation and the reaction occurs at +0.00 V (vs. Ag/AgCl). The method of the sensor construction is very simple and the sensor performed well, giving high sensitivity, high stability, and a broad detection range. The sensitivity of this system is 2.54 μA mM−1 and the limit of detection (S/N = 3) is 10 μM. A linear range was observed between 10 μM and 1000 μM of NADH (R2 = 0.993), which is preferable to that of the previous studies. The Fe2O3/CB electrode also oxidizes NADPH under the same condition and can be applied as an NADPH sensor. Moreover, when the sensor system was integrated into a dehydrogenase-based sensor system, it also showed a good sensing performance.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 5, 15 January 2010, Pages 1160–1165