کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868826 | 909814 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Aptamer based surface enhanced Raman scattering detection of vasopressin using multilayer nanotube arrays Aptamer based surface enhanced Raman scattering detection of vasopressin using multilayer nanotube arrays](/preview/png/868826.png)
Here we present an optofluidic surface enhanced Raman spectroscopy (SERS) device for on-chip detection of vasopressin using an aptamer based binding assay. To create the SERS-active substrate, densely packed, 200 nm diameter, metal nanotube arrays were fabricated using an anodized alumina nanoporous membrane as a template for shadow evaporation. We explore the use of both single layer Au structures and multilayer Au/Ag/Au structures and also demonstrate a facile technique for integrating the membranes with all polydimethylsiloxane (PDMS) microfluidic devices. Using the integrated device, we demonstrate a linear response in the main detection peak intensity to solution phase concentration and a limit of detection on the order of 5.2 μU/mL. This low limit of detection is obtained with device containing the multilayer SERS substrate which we show exhibits a stronger Raman enhancement while maintaining biocompatibility and ease or surface reactivity with the capture probe.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 5, 15 January 2010, Pages 1240–1243