کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869185 909824 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new protein A assay based on Raman reporter labeled immunogold nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A new protein A assay based on Raman reporter labeled immunogold nanoparticles
چکیده انگلیسی

A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG–AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO2 stretching of DTNB at 1333 cm−1 versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 2, 15 October 2008, Pages 178–183
نویسندگان
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