Multienzymatic system immobilization in sol–gel slides: Fluorescent superoxide biosensors development

One of the potential areas of research in the development of biosensors is the production of analytical devices based on the use of immobilized multienzymatic systems. In this work, we report the development of three analytical systems for superoxide radical detection using sol–gel technology to immobilize enzyme systems. These systems are based on the connected reactions of three enzymes (xanthine oxidase, superoxide dismutase and horseradish peroxidase) coupled to the probe Amplex red. The difference between these three systems lies in the immobilization of two or three enzymes into a single or in different sol–gel slides. We check the potential use of each designed systems to quantify superoxide radical and potential evaluation of radical scavenging properties of several antioxidant compounds.