کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869380 909829 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel immobilization strategy using oligonucleotide as linker for small molecule microarrays construction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel immobilization strategy using oligonucleotide as linker for small molecule microarrays construction
چکیده انگلیسی

A novel immobilization method based on oligonucleotide as linker has been developed for small molecule microarrays (SMMs) construction. The oligonucleotide tail was employed as a linker in solid-phase synthesis. Small molecules could be easily conjugated at the 5′ end of the oligonucleotide, previously modified with a functional group. Being a reactive species, the oligonucleotide was activated by UV irradiation, for the attachment of the conjugate to the slide surface. The method was successfully applied to structurally distinct small molecules, including biotin, antibiotic and drug. This immobilization strategy showed high efficiency, 1.1 fmol of small molecules in the spotting solution per spot gave a detectable signal (mean S/N = 10.9). The results suggest that it is very promising for exploring interaction between small molecules and proteins, and high throughput detecting the chemical compounds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 23, Issue 11, 15 June 2008, Pages 1694–1700
نویسندگان
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