کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869406 909830 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The orientationally controlled assembly of genetically modified enzymes in an amperometric biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The orientationally controlled assembly of genetically modified enzymes in an amperometric biosensor
چکیده انگلیسی

A novel, nitroreductase (NTR) containing a sequence of six cysteine amino acids, enabling strong thiolate bonds to form on a gold electrode surface without the loss of enzyme activity, was genetically engineered. The enzyme was directly immobilised at a gold electrode without the need for pre-treatment of the surface with a self-assembled monolayer or a conducting polymer. The ensemble was used to develop an amperometric biosensor for the detection of explosives containing nitroaromatic compounds. Preliminary results demonstrate detection levels down to 100 parts per trillion, signifying tremendous promise towards an in situ sensor for the detection of explosives.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issue 12, 15 June 2007, Pages 2869–2875
نویسندگان
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