کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869565 909833 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum
چکیده انگلیسی

This work describes an electrochemical method for the determination of the nitrate and nitrite reductase activities of Rhizobium japonicum. The advantage of the method lies in the use of whole cells for the analysis and we earlier developed this protocol for the assay of NO. The results obtained are comparable to the spectrophotometric Griess assay. As the method is based on electrochemical reduction, the commonly interfering biological components like ascorbic acid, uric acid, dopamine, etc., will not interfere with the analysis. This method can be extended to the fabrication of biosensors for nitrate and nitrite using the same principle.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 12, 15 August 2009, Pages 3487–3491
نویسندگان
, , , , , ,