کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869785 909838 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zirconium immobilized on gold–mercaptopropionic acid self-assembled monolayer for trace determination of phosphate in blood serum by using CV, EIS, and OSWV
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Zirconium immobilized on gold–mercaptopropionic acid self-assembled monolayer for trace determination of phosphate in blood serum by using CV, EIS, and OSWV
چکیده انگلیسی

Preparation, characterization, and application of a new sensor for the determination of phosphate in blood serum is described by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and osteryoung square wave voltammetry (OSWV) in the presence of an appropriate redox reaction probe. The sensor was based on ionic adsorption of phosphate on Zr(IV) immobilized on gold–mercaptopropionic acid self-assembled monolayer (Au–MPA–Zr(IV) SAM) electrode. Parameters influencing the method were optimized. A linear range calibration curve from 1.0 × 10−7 to 1.0 × 10−6 M PO43− with a detection limit of 5.30 × 10−8 M and mean of relative standard deviations (R.S.D.) of 2.75% for n = 4 was observed in the best conditions by OSWV. Possible interferences from the coexisting ions were also investigated. The results demonstrated that sensor could be used for the determination of phosphate in the presence of various ions. Regeneration of the surface was carried out successfully by 5-min sonication in 0.1 M KOH solution and then 1-min incubation in 1.0 × 10−3 M Zr(IV) with a good reproducibility, R.S.D. = 1.47% for n = 4 by OSWV. The validity of the method and applicability of the sensor were successfully tested by detection of phosphate in blood serum after deproteinization of sample without interference from sample matrix. The long-term storage stability of the electrode was studied. The experimental data is presented and discussed from which the new sensor is characterized.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 7, 15 March 2009, Pages 2199–2204
نویسندگان
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