کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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869798 | 909838 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol–gel network with gold nanoparticles Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol–gel network with gold nanoparticles](/preview/png/869798.png)
We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol–gel material with the ion-exchange capacity sol–gel to coimmobilize the Ru(bpy)32+ and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol–gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)32+ and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)32+ film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)32+ film to promote the electron transfer between Ru(bpy)32+ and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 μM to 5.2 mM with a detection limit of 12 nM.
Journal: Biosensors and Bioelectronics - Volume 24, Issue 7, 15 March 2009, Pages 2273–2276