کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
870072 | 909848 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A cowpea mosaic virus nanoscaffold for multiplexed antibody conjugation: Application as an immunoassay tracer
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A cowpea mosaic virus nanoscaffold for multiplexed antibody conjugation: Application as an immunoassay tracer A cowpea mosaic virus nanoscaffold for multiplexed antibody conjugation: Application as an immunoassay tracer](/preview/png/870072.png)
چکیده انگلیسی
Cowpea mosaic virus (CPMV), an icosahedral 30 nm virus, offers a uniquely programmable biological nanoscaffold. This study reports initial optimization of the simultaneous modification of two CPMV mutants with AlexaFluor® 647 fluorescent dyes and either IgG proteins or antibodies at specific sites on the virus scaffold. The capacity of CPMV as a simultaneous carrier for different types of molecules was demonstrated, specifically, when applied as a tracer in direct and sandwich immunoassays. The ability to label the virus capsid with antibody and up to 60 fluorescent dyes resulted in an improved limit of detection in SEB sandwich immunoassays, when used as a tracer, relative to a mole equivalent of dye-labeled antibody.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 8, 15 February 2006, Pages 1668–1673
Journal: Biosensors and Bioelectronics - Volume 21, Issue 8, 15 February 2006, Pages 1668–1673
نویسندگان
Kim E. Sapsford, Carissa M. Soto, Amy Szuchmacher Blum, Anju Chatterji, Tianwei Lin, John E. Johnson, Frances S. Ligler, Banahalli R. Ratna,