کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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870083 | 909849 | 2008 | 5 صفحه PDF | دانلود رایگان |
The design of an electrochemical glucose sensing device formed by the electrodeposition of multifunctional Au nanoparticles is reported here as a novel concept for an enhanced generic sensing platform. Initially gold nanoparticles (Au) were alternatively coated with a layer of positively charged redox polymer (ORP) and a negatively charged glucose oxidase (GOX) layer alternatively using layer-by-layer methodology to form multifunctional Au/ORP/GOX/ORP particles. The modification and stability of the Au nanoparticles was monitored by using UV–vis spectroscopy and zeta-potential measurements. The modified Au nanoparticles were electrophoretically deposited onto an electrode to produce an electrochemical glucose sensing device. A considerable influence of electrophoretic deposition time and potential was found on the sensing platform response. Preliminary responses to glucose addition showed an enhanced performance by applying an electrophoretic deposition potential of +1.2 V vs. Ag/AgCl for 30 min. The observed response in the case of microelectrode geometry was in the range of mA cm2. This work also shows that the presence of a second outer ORP layer on the functionalised Au nanoparticles improved the response.
Journal: Biosensors and Bioelectronics - Volume 24, Issue 1, 15 September 2008, Pages 55–59