کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870239 909856 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7
چکیده انگلیسی

In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issue 7, 15 February 2007, Pages 1550–1555
نویسندگان
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