کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870340 1470993 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform
چکیده انگلیسی

Determination of cholesterol level in blood is important in clinical applications. In this work, modified Au nanowires—electrochemical biosensor based on MEMS micro-fluidic platform is proposed for estimating total cholesterol in blood. This sensor consists of “aligned” Au nanowires as working electrode, platinum counter electrode deposited on the silicon platform and Ag/AgCl (3 M KCl) reference electrode. The “aligned” Au nanowires are immobilized with cholesterol oxidase and cholesterol esterase using specific covalent chemistry. Further, Au nanowires promotes better electron transfer between the enzymes and electrodes, because of their large surface to volume ratio, small diffusion time, large electrical conductivity and their aligned nature. The modified Au nanowires showed a stable calibration line and a quasi-linear relationship between cholesterol level and current response in the range of 1–6 mM (in steps of 1 mM over the baseline blood serum). The sensitivity of the modified electrode was found to be about 69 nA/mM with good storage and interference stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issues 9–10, 15 April 2007, Pages 2289–2294
نویسندگان
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