کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
870562 | 1471021 | 2016 | 5 صفحه PDF | دانلود رایگان |
• Fabrication of enzyme-carrying polymeric nanofibers by electrospinning.
• PVA nanofibers structure and size were not affected by the addition of enzyme.
• The electrospun CGTase/PVA nanofibers show excellent immobilisation efficiency.
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.
Journal: Biotechnology Reports - Volume 10, June 2016, Pages 44–48